Coding

Part:BBa_K1732022:Design

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-18)


EGFPCO-His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


Design Notes

Codon optimized for E.coli and His tag allows for easier protein purification.


Source

BBa_1491002

References